Abstract
Chicken serum insulin-like growth factor-1 (IGF-1) was extracted with an acid ethanol solution and submitted to a heterologous radioimmunoassay (RIA) after lyophilization. In the RIA, recombinant human IGF-1 (rhIGF-1) was used for standards and radioactive ligand. Anti-rhIGF-1 antiserum used was provided by the NIDDK. The inhibition curves for extracts of two-fold serial dilutions of chicken sera were essentially parallel to those for the standard and human sera. The recovery of rhIGF-1 added to chicken sera was 100.1±0.7% (n=30). The intrrassay and interassay variability (CV) for the RIA was 6.9% (n=10) and 7.6% (n=3), respectively. The limit of sensitivity for measurement of IGF-1 extracted from chicken serum was 5 pg/assay tube. Hypophysectomy significantly reduced serum levels of IGF-1 by 80% as compared to sham-operated control chicken. Dwarfing gene (dw) also significantly reduced serum IGF-1 by 89% compared to normal control. These results indicate that the present heterologous RIA was validated for measurement of immunoreactive IGF-1 levels in chicken serum.
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