Abstract

RATIONALE: ‘Blocking’ IgG antibodies induced by allergen immunotherapy can inhibit IgE-facilitated allergen binding to B-cells in a simple flow-cytometric assay. (Wachholz,P. et al., JACI 2003,12:915-922). We aimed to validate this assay according to the guidelines from the International Conference on Harmonisation (ICH) to determine its value in monitoring successful immunotherapy.

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