Abstract
In the associated main paper (“Labeled substance P as a neuropeptide reporter substance for enzyme activity” (http://doi.org/10.1016/j.jpba.2019.112953)), substance P was shown to be a valuable neuropeptide reporter substance to monitor the protease activity of serum. The assay was developed based on the predecessor assay using bradykinin (“A vote for robustness: Monitoring serum enzyme activity by thin-layer chromatography of dabsylated bradykinin products”, http://doi.org/10.1016/j.jpba.2017.06.007). Both neuropeptides are of interest in inflammation and pain research and were thus explored for use with capillary blood and blood cards (see associated MethodX paper “Neuropeptide reporter assay for serum, capillary blood and blood cards”). Here, we present validation data for the assay when sampling with blood cards as well as data on the use of fresh capillary blood.
Highlights
Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards
Both neuropeptides are of interest in inflammation and pain research and were explored for use with capillary blood and blood cards
Analytical Chemistry Protease activity in capillary blood is studied by observation of the fragments of neuropeptide reporter substances Table Image Chart Figure Thin-layer chromatography and image analysis Scanner: Canon 9000F Mark2 Software: Photoshop plug-in Silver Efex Pro (Google, Mountain View, USA), JustTLC (Sweday, Sodra Sandby, Sweden) Raw and analysed data in Excel The protocol is available in the associated MethodX article “Neuropeptide reporter assay for serum, capillary blood and blood cards”
Summary
Core Unit Proteomics, Interdisciplinary Center for Clinical Research, Medical Faculty, University of Münster, Germany article info. 1016/j.jpba.2019.112953)), substance P was shown to be a valuable neuropeptide reporter substance to monitor the protease activity of serum. The assay was developed based on the predecessor assay using bradykinin Both neuropeptides are of interest in inflammation and pain research and were explored for use with capillary blood and blood cards (see associated MethodX paper “Neuropeptide reporter assay for serum, capillary blood and blood cards”).
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