Abstract
BackgroundFusarium crown rot (FCR) is a chronic and severe disease in cereal production in semi-arid regions worldwide. A putative quantitative trait locus conferring FCR resistance, Qcrs.cpi-1H, had previously been mapped on the long arm of chromosome 1H in barley.ResultsIn this study, five pairs of near-isogenic lines (NILs) targeting the 1HL locus were developed. Analysing the NILs found that the resistant allele at Qcrs.cpi-1H significantly reduced FCR severity. Transcriptomic analysis was then conducted against three of the NIL pairs, which placed the Qcrs.cpi-1H locus in an interval spanning about 11 Mbp. A total of 56 expressed genes bearing single nucleotide polymorphisms (SNPs) were detected in this interval. Five of them contain non-synonymous SNPs. These results would facilitate detailed mapping as well as cloning gene(s) underlying the resistance locus.ConclusionNILs developed in this study and the transcriptomic sequences obtained from them did not only allow the validation of the resistance locus Qcrs.cpi-1H and the identification of candidate genes underlying its resistance, they also allowed the delineation of the resistance locus and the development of SNPs markers which formed a solid base for detailed mapping as well as cloning gene(s) underlying the locus.
Highlights
Fusarium crown rot (FCR) is a chronic and severe disease in cereal production in semi-arid regions worldwide
Similar to those noticed in wheat [14, 15], strong interactions between FCR severity and other characteristics including flowering time [12, 16] and plant height [11, 17] have been detected in barley
Development and validation of Near-isogenic line (NIL) targeting the FCR resistance locus on 1HL Eight heterozygous plants were initially selected from the two segregating populations based on the profiles of the SSR marker WMC1E8
Summary
Fusarium crown rot (FCR) is a chronic and severe disease in cereal production in semi-arid regions worldwide. Fusarium crown rot (FCR), caused mainly by F. pseudograminearum, is a severe and chronic disease of cereals in semi-arid cropping regions worldwide [1, 2]. To reduce FCR damage, several agronomic measures have been developed They include crop rotation and stubble management [3, 4]. Four putative QTL conferring FCR resistance have been reported in barley [9] They locate on chromosome arms 1HL [10], 3HL [11], 4HL [12] and 6HL [13], respectively. Results from previous studies showed that water availability affects FCR development [19]
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