Abstract
A simple, rapid, and sensitive isocratic reversed-phase LC method using a monolithic column has been developed and validated for simultaneous analysis of the active components of silymarin [taxifolin, silydianin, silychristin, diastereomers of silybin (silybin A and B), and diastereomers of isosilybin (isosilybin A and B)] and dimethyl-4,4′-dimethoxy-5,6,5′,6′-dimethylene dioxybiphenyl-2,2′-dicarboxylate in a commercial formulation. The mobile phase was a 45:55 (v/v) mixture of methanol and 5 mM NaH2PO4 (adjusted to pH 2.75 with phosphoric acid) at a flow rate of 1.5 mL min−1. UV detection was performed at 288 nm and quantification was based on peak area. The method was validated for linearity, accuracy, precision, selectivity, and robustness.
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