Abstract
A reliable simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for estimation of lafutidine (LF) and domperidone (DP) in combined tablet dosage forms. The analysis was performed on 10×20 cm aluminium-precoated plates coated with 0.2 mm layers of silica gel 60 F 254 (E-Merck, Germany) with ethyl acetate:methanol:toluene:acetone:glacial acetic acid (1.0:1.5:4.0:2.0:0.2 v/v/v/v/v) as mobile phase. Camag TLC Scanner III was used for the UV densitometric scanning at 285 nm. This system was found to give a compact spot of LF and DP at retention factor (R f ) value of 0.32±0.03 and 0.65±0.02 respectively. Linear regression analysis data for the calibration curve showed good relationship with respect to peak area in the concentration 100-500ng per spot for both LF and DP with (r 2 = 0.9993) and (r 2 = 0.9985) for LF and DP respectively. Limit of detection (15.51 ng/spot for LF and 23.04 ng/spot for DP), limit of quantication (47.03 ng/spot for LF and 69.83 ng/spot for DP) were found. The proposed HPTLC method has potential applications in determination of lafutidine and domperidone in tablet formulations.
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