Abstract
A highly validated and selective high performance thin layer chromatography (HPTLC) method was developed for the determination of loratadine (LOR) and desloratadine (DES) in their pharmaceutical formulations. The proposed method was based on HPTLC separation of the two drugs followed by densitometric measurements of their spots at 254 nm. The separation was carried out on Merck HPTLC aluminum sheets of silica gel 60 F254 using methanol-ammonia (10:0.3, ν/ν) as mobile phase. The linear regression analysis data were used for the regression line in the range of 0.25–0.85 and 0.10–1.00 μg band−1 for LOR and DES, respectively. The method was successfully applied to the analysis of the two drugs in their commercial tablets, capsules, and syrups, and the results were in good agreement with those obtained with the comparison method. The proposed method is specific for the simultaneous determination of loratadine and desloratadine in the presence of other co-formulated drugs, such as pseudoephedrine. The method is...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have