Validated high performance thin layer chromatographic method for simultaneous quantification of major iridoids in Vitex trifolia and their antioxidant studies

Abstract

Negundoside ( 1), agnuside ( 2) and 6′- p-hydroxy benzoyl mussaenosidic acid ( 3) are known bioactive metabolites in Vitex trifolia. In the present study a simple precise and reproducible method was developed for simultaneous quantitation of NS ( 1), AS ( 2) and HMA ( 3) and the antioxidant capacity of above markers has also been determined. Marker compounds have been resolved using silica gel 60 F 254 plates, petroleum ether (60–80)/toluene/acetone/water (10:10:80:2 v/v/v/v) as the mobile phases. The method does not employ any derivatisation procedure and can be used as a quality control tool for routine analysis of drugs V. trifolia and V. negundo together with their commercial extracts. NS ( 1), AS ( 2) and HMA ( 3) showed significant activity in DPPH and NO radical scavenging assays.