Abstract
In the current study, we employed high-resolution proton and carbon nuclear magnetic resonance spectroscopy (1H and 13C NMR) for quantitative analysis of glycerol in drug injections without any complex pre-treatment or derivatization on samples. The established methods were validated with good specificity, linearity, accuracy, precision, stability, and repeatability. Our results revealed that the contents of glycerol were convenient to calculate directly via the integration ratios of peak areas with an internal standard in 1H NMR spectra, while the integration of peak heights were proper for 13C NMR in combination with an external calibration of glycerol. The developed methods were both successfully applied in drug injections. Quantitative NMR methods showed an extensive prospect for glycerol determination in various liquid samples.
Highlights
Glycerol is a simple polyol compound with three hydroxyl groups that are responsible for its solubility in water, hygroscopic, and hyperosmolar nature
D2O showed the presence of ethylene signal
The results showed that the minimum relative errors at two concentrations were 0.004 and 0.014, revealing that peak height integration and external calibration calculation method were alternative for 13 C Nuclear magnetic resonance (NMR) analysis
Summary
Glycerol is a simple polyol compound with three hydroxyl groups that are responsible for its solubility in water, hygroscopic, and hyperosmolar nature. As a potent osmotic dehydrating agent, glycerol has been used in medicinal and pharmaceutical preparations with additional effects on stroke, head injury, brain edema and glaucoma to reduce elevated tissue pressure [1,2,3,4]. Appropriate analytical methods are needed in order to control the officially required specifications of glycerol. Any compound containing a couple of neighbor hydroxyls or phenolic hydroxyl is able to participate in a redox reaction, resulting in unsatisfactory specificity and accuracy of this method. Analytical chromatography hyphenated with different detections has been developed for glycerol determination in drug injections.
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