Abstract
Abstract . This research was aimed to validation analytical method of Saccarine and to determine saccarine consentration in soft drink High Performance Liquid Chromatography. Sodium Saccarine salts are used as standard. Sodium Saccarine salts dissolve in mobile phase methanol : fosfat buffer (60:40). Concentration of standard saccarine solution made in 20, 40, 60, 80, and 100 ppm . HPLC Agilent Technology 1260 Infinitely Series , Poroshell/C18 column - 50 mm lenght- id 4 . 6 mm - particles size 2 . 7 µm , UV-detector in λ 265 nm, injection volume 20 µL , and flow rate 1 mL/minutes were used in this research. Method validation parameters were determined by linierity, precision, accuracy, LOD, LOQ, and selectivity. The results show that lineariy with R = 0 . 998702 , precision with RSD = 0 . 192%, accuracy with % recovery = 100 . 26%, LOD = 0 . 172 ppm, LOQ = 0 . 573 , and selectivity with R s = 0.2. Testing for the ‘Sprite Zero’ soft drink show that 117 . 6 mg/kg saccarine (under the maximum content in carbonated/noncarbonated soft drink). Conclution from this research is, HPLC method can use in analysis saccarine content in soft drink Kata kunci: metode penentuan, sakarin, kromatografi kinerja tinggi
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
