Vaginal cytology, vaginoscopy and progesterone profile: breeding tools in bitches

K S Rao,K C S Reddy,K G S Raju

doi:10.33899/ijvs.2011.5656

Abstract

The exfoliative vaginal cytology, vaginoscopic examination of vaginal mucosa and progesterone profiles were recorded in an attempt to identify the ideal time of breeding in bitches. A total of 18 anestrus bitches were selected and divided into 03 groups (Control, CABG and eCG groups). The bitches in control group did not receive any treatment and exhibited estrus. The estrus was induced with Cabergoline (CABG) and equine Chorionic Gonadotropin (eCG) in the other two groups of bitches. In control group, higher percentage of superficial cells (89.94 ± 0.64) and lower percentage of intermediate (7.30 ± 0.77) and parabasal cells (2.76 ± 0.30) were characteristic vaginal cytological changes during estrus. Vaginoscopic examination of CABG group of bitches revealed that the vaginal mucus was creamy and paper white with angular shrinkage during estrus. In eCG group of bitches, the plasma progesterone concentration was 1.55 ± 0.19 ng/ml on day 8.00 ± 0.71 of proestrus. The conception rates were 66.66, 83.33 and 83.33 per cent in Control, Cabergoline and eCG groups, respectively. The litter size varied from 3.50 + 1.12 to 4.83 + 0.83 in the three groups.

Highlights

There is a considerable variation in the time of ovulation in relation to the onset of serosanguinous discharge of early proestrus in bitches
The present study is aimed to suggest breeding time based on vaginal cytology, vaginoscopy and progesterone profile
CABG Group: Six bitches were subjected to induction of estrus by using Cabergoline (Cabgol, Sun Pharmaceutical Industries, Mumbai, India) at a dose of 05 μg / kg body weight orally until the detection of proestrual bleeding. equine Chorionic Gonadotropin (eCG) Group: Six anestrus bitches were subjected to induction of estrus with the eCG (Folligon- Intervet International, Boxmeer, Netherland) at a dose of 20 IU/kg body weight I/M for 05 consecutive days and on day 05 injected with hCG (Chorulon - Intervet International, Boxmeer, Netherland) at a dose of 500 IU I/M

Summary

Introduction

There is a considerable variation in the time of ovulation in relation to the onset of serosanguinous discharge of early proestrus in bitches. The present study is aimed to suggest breeding time based on vaginal cytology, vaginoscopy and progesterone profile. The vaginal smears were collected every alternate day from control whenever the bitches entered into proestrus till the day of diestrus for the purpose of determining the stage of estrous cycle.

Results

Conclusion