Abstract
Abstract Dimorphic pathogenic fungus Penicillium marneffei can cause penicilliosis which is a common infectious disease in AIDS patients in Southeast Asia and southern parts of China. Mp1p is a highly antigenic cell wall mannoprotein encoded by MP1 gene of P. marneffei and it is a pivotal factor for the pathogen infection and survival. Recombinant protein Mp1p produced in E.coli and Pichia pastors was used as an antigen vaccine against penicilliosis. While administered with different adjuvants and injection routes to BALB/c mice, this vaccine protein combination with Sigma Adjuvant System by intraperitoneal injection elicited a strong immunoglobulin G antibody response and conferred protection against a lethal P. marneffei challenge. These results showed that protein Mp1p could protect BALB/c mice in a dose, adjuvant and injection dependent manner. Protein vaccine Mp1p induced resistance was assessed in terms of survival, fungal growth (CFU) and histopathological examination. Immunised BALB/c mice could be protected against P. marneffei with 100% survival. CFU and histopathological analysis implied that fungal growth was systemically restricted at target organ of vaccinated mice, especially in liver where there was a significant lower value of CFU than non-vaccinated mice. Our results support the further development of an effective vaccine against penicilliosis.
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