Vaccine Development Strategy for Myiasis Caused by the Larvae of Chrysomya Bezziana (The Old World Screwworm Fly)

Abstract

Indonesia is an endemic area for myasis caused by the larvae of Chrysonrya bezziana . So far, the controlling method for myiasis is using insecticides such as organophosphate and coumaphos compounds . Sterile Insect Technique (SIT) was a successful method for myiasis control, however, this method is expensive, beside it needs a large amount of insecticide. Therefore, vaccinations as an alternate controlling method for myiasis which relatively cheap, environment friendly and in accordance with the sustainable agriculture concept are needed . The strategy of myiasis vaccine development has been done by the Indonesia Research Institute for Veterinary Science in collaboration with IUC-ITB, Bandung and CSIRO, Brisbane-Australia, are briefly discussed in this paper. The first step was to identify and purify the protective antigens from excretory/secretory material and peritrophic membrane. The excretory/secretory material was purified and analysed using amino-terminal sequencing and resulted trypsin and chymotrypsin at 26 kDa and 28 kDa, respectively . The peritrophic membrane was then fractionated and resulted candidate antigens of Cb 15, Cb42 and Cb48 . The three antigens were expressed into bacterial Eschericia coli and yeast Pichia pastoris resulting different yield depending on each protein character . The strategy of myiasis vaccine could be used as an alternate way for vaccine development for other parasite diseases in Indonesia. Key words: Chrysoniya bezziana, vaccine, excretory/secretory, peritrophic membrane