Abstract

Influenza is a severe viral disease, a frequent complication of which is a secondary bacterial pneumonia. Influenza vaccines prevent secondary bacterial complications. Virus-like particles are one of the promising areas for the development of new vaccines. The aim of this work is to study the correlation of the pathomorphological characteristics of the lungs with clinical, virological, and microbiological markers of the disease at vaccination with virus-like particles (VLPs), containing hemagglutinin (HA) of influenza virus (HA-Gag-VLPs) in a murine model of secondary bacterial pneumonia induced by S. pneumoniae after influenza infection. BALB/c mice were vaccinated with VLPs containing influenza HA. After 21 days, mice were infected with two strains of influenza viruses, homologous and non-homologous, and 5 days after viral infection, were infected with S. pneumoniae. The vaccination effect was evaluated by morphological, virological (titer of the virus in the lungs) and microbiological (titer of bacteria in the lungs) data, and was confirmed by clinical data (survival, change in body weight). Immunization with HA-Gag-VLPs, followed by infection with a homologous influenza virus and S. pneumoniae, reduced the area of foci of inflammation, inhibited the replication of the virus and bacteria in the lungs, and also protected animals from death and reduced their weight loss. Immunization with HA-Gag-VLPs upon infection with a heterologous strain and S. pneumoniae did not affect these criteria. The immunization with HA-Gag-VLPs prevented the viral replication, providing a reduction of S. pneumoniae titer and the degree of lung damage, protecting animals from the disease in a murine model of secondary bacterial pneumonia, induced by S. pneumoniae, after influenza infection with homologous strain of the virus.

Highlights

  • Influenza is a severe viral disease, a frequent complication of which is a secondary bacterial pneumonia

  • Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data

  • The aim of this work is to study the correlation of the pathomorphological characteristics of the lungs with clinical, virological, and microbiological markers of the disease at vaccination with virus-like particles (VLPs), containing hemagglutinin (HA) of influenza virus (HA-Gag-VLPs) in a murine model of secondary bacterial pneumonia induced by S. pneumoniae after influenza infection

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Summary

ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ

Экспрессирующими гемагглютинин, на развитие постгриппозной бактериальной пневмонии у мышей: патоморфологические, вирусологические, микробиологические и клинические данные. Ю.1, 2, Поддубиков А.В.1, Вартанова Н.О.1, Карташова Н.П.1, Глубокова Е.А.1, Мхитаров В.А.3, Джалилова Д.Ш.3, Макарова О.В.3, Ленева И.А.1. Цель настоящей работы – изучение корреляции патоморфологической характеристики лёгких с клиническими, вирусологическими, микробиологическими маркерами заболевания при вакцинации ВПЧ, несущими гемагглютинин вируса гриппа (HA-Gag-ВПЧ), на модели вторичной бактериальной пневмонии мышей, вызванной S. pneumoniae, после гриппозной инфекции, индуцированной гомологичным и гетерологичным штаммами вируса гриппа. В экспериментальной мышиной модели вторичной бактериальной пневмонии, вызванной S. pneumoniae, после гриппозной инфекции индукция иммунного ответа к НА вируса гриппа при парентеральной иммунизации мышей HA-Gag-ВПЧ способна предотвращать размножение вируса, обеспечивая снижение бактериальной нагрузки S. pneumoniae и степень поражения лёгких, защищая животных от заболевания при антигенном соответствии вакцины и штамма вируса гриппа, используемого для провокации бактериальной инфекции. – микробиологическая часть исследования; Мхитаров В.А., Джалилова Д.Ш., Макарова О.В.

ORIGINAL RESEARCH
Introduction
Материал и методы
Valid Медиана
Findings
Дни после заражения

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