V1 Interpatch Projections to V2 Thick Stripes and Pale Stripes

Abstract

Cytochrome oxidase (CO) reveals two compartments in V1 (patches and interpatches) and three compartments in V2 (thin, pale, and thick stripes). Previously, it was shown that thin stripes receive input predominantly from patches. Here we examined the projections to thick and pale stripes in macaques, revealed by retrograde tracer injections. After thick stripe injection, cells were distributed in layer 2/3 (67%), layer 4A (7%), layer 4B (23%), and layer 5/6 (2%). Except in layer 5/6, cells were concentrated in interpatches, with a stronger bias in layer 2/3 (84%) than in layer 4B (75%). After pale stripe injection, cells were found in layer 2/3 (87%), layer 4A (2%), layer 4B (10%), and layer 5/6 (2%). As for thick stripes, cells were located preferentially in interpatches in layer 2/3 (84%) and layer 4B (72%) but not in layer 5/6. Thick stripes received a higher proportion of their input from layer 4B, compared with pale stripes, consistent with reports that thick stripe neurons exhibit a pronounced layer 4B influence. This difference aside, both stripe types receive similar inputs from V1, at least in terms of cortical layer and CO compartment. This finding was bolstered by injecting different tracers into pale and thick stripes; 10-27% of cells were double labeled, with most located in interpatches. These results suggest that the distinctive receptive field properties of neurons in thick and pale stripes are generated by local V2 circuits, or by other specific projections, rather than by differing sources of laminar and compartmental input from V1.