UV-B辐射增强和O3浓度升高对大豆（Glycine max.）叶片内源激素和抗氧化能力的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 UV-B辐射增强和O3浓度升高对大豆叶片内源激素和抗氧化能力的影响 DOI: 10.5846/stxb201306081450 作者: 作者单位: 沈阳农业大学 农学院,沈阳农业大学 农学院,沈阳农业大学 农学院,沈阳农业大学 农学院,沈阳农业大学 农学院,沈阳农业大学 农学院 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金(30970448);辽宁省高等学校优秀人才支持计划(LJQ2011072) Effects of plant endogenous hormones and antioxidant ability in soybean leaves under UV-B and ozone stress Author: Affiliation: College of agronomy,Shenyang Agricultural University,College of agronomy,Shenyang Agricultural University,College of agronomy,Shenyang Agricultural University,College of agronomy,Shenyang Agricultural University,College of agronomy,Shenyang Agricultural University,College of agronomy,Shenyang Agricultural University Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:揭示大豆叶片内源激素对UV-B和臭氧胁迫的代谢机制和响应方式,为从分子水平研究植物内源激素对UV-B(Ultraviolet-B)和O3(Ozone)胁迫的适应机制奠定基础。以大豆(Glycine max.)为试验材料,利用开顶式气室研究UV-B(0.32 W/m2)和O3((110±10) nmol/mol)复合胁迫对大豆叶片内源激素含量及活性氧代谢系统的影响。结果表明:在大豆整个生育期内,与对照相比,UV-B胁迫使大豆叶片ABA(Abscisic acid)含量、ZR(Zeatin riboside)含量和IAA(Indoleacetic acid)含量显著降低,IAA/ABA、ZR/ABA、(IAA+ZR)/ABA比值升高,O2·(Superoxide anion free radical,O2·)产生速率和MDA(Malonaldehyde)含量升高,SOD(Superoxide dismutase)、CAT(Catalase)和POD(Peroxidase)活性显著降低;高浓度O3胁迫下,大豆叶片ABA和IAA含量显著下降、ZR含量显著增加,IAA/ABA、ZR/ABA、(IAA+ZR)/ABA值显著升高,O2·产生速率和MDA含量增加,SOD、CAT和POD活性显著降低;UV-B和O3复合胁迫下,大豆叶片ABA含量、ZR含量和IAA含量降低,ZR/ABA、(IAA+ZR)/ABA值下降,而IAA/ABA值升高,O2·产生速率和MDA含量显著增加,SOD、CAT和POD活性显著降低。UV-B辐射增强和O3浓度升高单一及复合作用使大豆叶片内源激素间平衡改变,进而影响大豆叶片的代谢水平。持续胁迫下,植株抗氧化能力下降,对大豆表现为伤害效应。UV-B和O3复合胁迫比单独胁迫时的影响有所加深,但是小于两者单独作用时影响的简单累加。 Abstract:Widespread areas of crop production are presently at risk for damage from ambient atmospheric ozone (O3) concentrations and ultraviolet-B radiation (UV-B). The concentration of tropospheric ozone and UV-B have been rising due to aggravating urbanization since the Industrial Revolution, which has had a significant impact on plants and plants play an important role in adjusting structure and function of the ecosystem. Thus, to correctly evaluate how elevated O3 concentration and UV-B radiation affect plants is of great significance. The effects of O3 and UV-B on plant growth and some physiological indexes such as photosynthesis have been reported separately for a number of species by controlled experiments,but only few experiments have focused on their interaction. It is not distinct on interaction mechanism between O3 and UV-B. Especially, it is lack of reports to investigate the effects on endogenous hormone of a single species exposed to UV-B and O3 under well-controlled experimental conditions.Open-top chambers were utilized to investigate endogenous hormones content, change of lipid peroxidation extent, ROS (Reactive oxygen species, ROS) production rate, anti-oxidation enzymes activities in soybean leaves under elevated ozone concentration (110±10 nmol/mol) and UV-B radiation (0.32 W/m2) singly and in combination treatment. The results indicated that enhanced UV-B radiation induced a decrease on abscisic acid (ABA) content, indoleacetic acid (IAA) content, zeatin riboside (ZR) content, the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and a increase on ratio of IAA/ABA, ZR/ABA, (IAA+ZR)/ABA, superoxide anion free radical (O2·) production rate, Malondialdehyde (MDA) content, in soybean leaves during the whole growth stage and the ratio of IAA/ABA, ZR/ABA, (IAA+ZR)/ABA showed significantly increase at flowering stage,but slightly decrease at podding stage. Elevated O3 concentration exposure showed the similar effects, which resulted in lower ABA content, IAA content, the activities of SOD, CAT, POD and higher ZR content, O2· production rate, MDA content, ratio of IAA/ABA, ZR/ABA, (IAA+ZR)/ABA compared with the control and the ratio of IAA/ABA, ZR/ABA, (IAA+ZR)/ABA showed significantly increase at branching stage and flowering stage. A increase of ZR content may improve the antioxidant ability of soybean. UV-B radiation and O3 concentration combined stress induced a decrease on ABA content, IAA content, ZR content, the activities of SOD, CAT, POD, ratio of ZR/ABA, (IAA+ZR)/ABA and a increase on IAA/ABA ratio, O2· production rate, MDA content. The ratio of ZR/ABA showed significantly decrease at flowering stage and podding stage, but the difference of the ratio of IAA/ABA and (IAA+ZR)/ABA is not significant at the whole growth stage. The antagonism effect appears between ABA and ZR under enhanced UV-B and O3 in combination. Obviously, enhanced UV-B radiation and elevated O3 concentration caused significant variation on endogenous hormone balance and accelerated ROS metabolism rates and reduced anti-oxidative enzymes activities in soybean leaves. Compared with UV-B and O3 singly, UV-B and O3 in combination caused significant decrease in soybean endogenous hormone content and antioxidant ability. UV-B and O3 in combination enhanced the negative effect on soybean compared with UV-B and O3 alone but less than the simple accumulation effects of UV-B and O3 alone. 参考文献 相似文献 引证文献