UV–VIS and HPLC studies on Amphiroa anceps (Lamarck) Decaisne

Abstract

The present study was aimed to explore phytochemical constituents present in Amphiroa anceps (Lamarck) Decaisne. The extracts of A. anceps were scanned in the wavelength ranging from 200 to 1100nm by using Shimadzu Spectrophotometer. HPLC method was performed on a Shimadzu LC-10 AT VP HPLC system, which was equipped with a model LC-10AT pump, UV–VIS detector SPD-10AT, Rheodyne injector fitted with a 20μl loop and auto injector SIL-10AT. Out of 156 (2×6×13=156) tests for the presence or absence of the above compounds, 42 tests gave positive results and the remaining 114 showed negative results. The 42 positive results showed the presence of alkaloids, steroids, phenolic groups, saponins, tannin, flavonoids, terpenoids, glycosides, proteins and sugars. The results of the ash analysis discovered the existence of sulphur, calcium, magnesium, iron, phosphorous and chlorine in all the extracts of A. anceps. The UV–VIS profile of A. anceps benzene extracts showed peaks at 670, 612, 536, 504, and 412nm with the absorption 2.004, 0.333, 0.417, 0.608 and 3.311. The UV–VIS profile of A. anceps chloroform extracts showed the peaks at 666, 608, 538, 502 and 416nm with the absorption 1.029, 0.39, 0.458, 0.552 and 2.648. The qualitative UV–VIS spectrum profile of A. anceps methanolic extracts showed peaks at 656 and 330nm with the absorption 0.295 and 3.656. The HPLC profile of A. anceps aqueous extracts showed two prominent peaks at a retention time of 1.737 and 2.680min and some moderate peaks were observed with the retention time 4.083, 6.387 and 1.490min. The qualitative HPLC fingerprint profile of isopropanol extract of A. anceps showed one prominent peak at a retention time of 2.673min. The HPLC profile of A. anceps methanolic extracts illustrated two prominent peaks at a retention time of 1.927 and 2.667min and some moderate peaks were also observed with the retention time 2.347, 4.077 and 5.873min, respectively.