Abstract
UV/Vis absorption spectroelectrochemistry is a very promising analytical technique due to the complementary information that is simultaneously obtained from electrochemistry and spectroscopy. In this work, this technique is used in a parallel configuration to study the oxidation of folic acid in alkaline medium. Herein, UV/Vis absorption spectroelectrochemistry has been used to detect both the oxidation products and the folic acid consumed at the electrode/solution interface, allowing us to develop an analytical protocol to quantify vitamin B9 in pharmaceutical tablets. Linear ranges of three orders of magnitude have been achieved in basic medium (pH = 12.9), obtaining high repeatability and low detection limits. The spectroelectrochemical determination of folic acid in pharmaceutical tablets at alkaline pH values is particularly interesting because of the changes that occur in the optical signal during the electrochemical oxidation of FA, providing results with very good figures of merit and demonstrating the utility and versatility of this hyphenated technique, UV/Vis absorption spectroelectrochemistry.
Highlights
Instrumental analytical techniques commonly used to identify and quantify a specific molecule are typically based on the use of a single signal characteristic of the compounds under study
It should be noted that electrochemistry is used to generate the oxidation product that, combined with spectroscopy, allows us to obtain a determination of this molecule
A double determination of the sample is possible, but sometimes, as in the system shown in this work, the electrochemical signal cannot be used for quantitative purposes, and the determination is made only with the spectroscopic data generated during the oxidation process of the folic acid (FA)
Summary
Instrumental analytical techniques commonly used to identify and quantify a specific molecule are typically based on the use of a single signal characteristic of the compounds under study. Since FA has a characteristic UV/Vis absorption spectrum and its oxidation process is previously described, UV/Vis-SEC can be a suitable instrumental technique to study the electron transfer reaction of vitamin B9.
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