UV-SPECTROPHOTOMETRIC ESTIMATION OF ACYCLOVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Abstract

Analytical method development being a vital part of pre formulation - formulation research and development obviates the need to develop reliable, effective, e co friendly and cost effective methodologies for routine analysis of active pharmaceutical ingredients. UV spectroscopy is one of the earliest, yet of wide applications in drug analysis in different stages of formulations and quality control; despite the availabilities of sophisti cated chromatographic techniques and other hyphenated techniques. Current research attempts to develop simple, sensitiv e, accurate, precise and economical UV spectrophotometric methods for the routine analysis of acyclovir in bulk and pharmaceutical dosage forms using two separate alkaline medi a , 0.1N NaOH (method A) and 0.1N KOH (method B) and validate them as per ICH gui delines. In both the methods maximum absorbance was observed at 264 nm. Beer’s law was obeyed in the concentration of 2.5 - 40 µg / mL in method A and 2.5 - 30 µg / mL in method B with correlation co efficient of 0.999. The % recovery carried out by adding know n amount of standard drug to pre - analyzed tablet solutions was 98.75 ± 0.52 % to 99.78 ± 0.69 % (method A) and 98.55 ± 0.31 % to 99.78 ± 0.22 % (method B). Intra and interday precision expressed in % RSD were 0.38 ± 0.01 and 0.27 ± 0.02 - 0.44 ± 0.01 respe ctively and the percent purity was 99.85 ± 0.05 %. The methods were validated statistically as per ICH guidelines and the results obtained were within the acceptance criteria for the param eters relating to linearity, accuracy, precision.