Abstract
The UV-enhancement of λ bacteriophage recombination has been examined utilizing parental phages labeled by means of host-controlled modification. Light UV - irradiation of λ phages infecting HCR + or HCR − Escherichia coli bacteria causes similar absolute increases in the frequencies of recombinants among all progeny phages and among progeny that contain HCM-labeled—λ · K12(P1)—parental DNA strands. The UV-induced recombination involves breakage and reunion of parental DNA strands. With increasing UV dose to the infecting phages there is a decrease in the relative yield of progeny that retain a parental HCM label. The HCM-labeled λ progeny recovered from multiple infections with UV-irradiated phages of excision-defective HCR −, but not HCR +, bacteria contain nearly as many host-cell reactivable UV lesions as the infecting phages; these HCM-labeled progeny probably contain mainly conserved parental DNA. The possibility that there may be a selection against recovery of semiconserved parental DNA from infections with UV-irradiated phages is discussed. UV-induced recombinants may be formed prior to replication of the infecting phage DNA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
