Abstract
As a natural product, chitosan has the inherent properties of being biocompatible, biodegradable, and non-toxic. These properties make chitosan an ideal candidate for inclusion in matrices designed for use in enzyme immobilization for clinical analysis. The current work demonstrates the feasibility of using chitosan in electrochemical biosensor fabrication. The enzyme, sulfite oxidase (SOD), was covalently immobilized onto the matrix of chitosan-poly(hydroxyethyl methacrylate) (chitosan-pHEMA), a natural/synthetic polymer hybrid material obtainable via a UV curing process. An electron transfer mediator, p-benzoquinone was coupled onto the polymer network for the activation of chitosan-pHEMA copolymer, after completion of the photo-induced polymerization reaction. Chitosan-pHEMA was characterized using Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) techniques. The biological activity of the immobilized SOD and the electroactivity of the coupled p-benzoquinone were examined.
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