Abstract
In order to evaluate the method of UV-B radiation damage on plant cell by photonic technology,soybean callus was radiated by 50 μW/cm2 UV-B for 16 h.Ultraweak photon emission from soybean callus was collected and analyzed.The results showed that strength of spontaneous luminescence(SL) in ultraweak photon emission of soybean callus increased and delayed luminescence(DL) decreased gradually.Through the analyses of dynamic presses of DL,the change rules of dynamic parameters of DL such as integrated inten-sity I(T),initial I0,correlation time τ and decay constant β were obtained.It was indicated that integrated intensity I(T),initial photon number I0,correlation time τ and decay constant β decreased with the UV-B radia-tion time,and I(T) was positive correlation to initial photon number I0.The order parameter used to describe order degree of cell was defined according to biological signification of ultraweak photon emission.The study found that the changes of the order parameter of soybean callus under the action of UV-B radiation reflected the damage of UV-B radiation on cell.So,the damage of UV-B radiation on plant cell could be expressed by the order parameter based on ultraweak photon emission of cell.
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