UV-B-absorbing compounds in modern Cedrus atlantica pollen: The potential for a summer UV-B proxy for Northwest Africa

Benjamin A Bell,Rachid Ilmen,Peter Ryan,Roy A Wogelius,William J Fletcher,Alistair Wr Seddon

doi:10.1177/0959683618777072

Benjamin A Bell, Rachid Ilmen + Show 4 more

Open Access

https://doi.org/10.1177/0959683618777072

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Abstract

Sporopollenin is a complex biopolymer which is the main component of the pollen grain exine and is partly composed of the aromatic compounds para-coumaric acid ( pCA) and ferulic acid (FA). These compounds absorb ultraviolet-B radiation (UV-B, 280–315 nm), and their abundance in pollen and spores has been shown to increase in response to increased UV-B flux. Here, we show that the relative abundance of UV-B-absorbing compounds (UACs) measured using Fourier Transform Infrared Spectrometry (FTIR) in modern pollen of autumn-pollinating Cedrus atlantica trees increases in response to summer UV-B flux. This relationship was observed in native Moroccan samples ( r2 = 0.84, p < 0.0001), but not across a larger environmental gradient including non-Moroccan samples ( r2 = 0.00, p = 0.99). For non-Moroccan samples of known provenance, the abundance of UACs is similar to the abundance of UACs found in samples from their place of origin. The FTIR spectra of these samples also closely resemble the FTIR spectra of samples from their place of origin. This unexpected finding suggests there could be a heritable component to UAC production possibly associated with epigenetic memory, an important adaptive mechanism in conifers. Our results indicate that the relative abundance of UACs in Cedrus atlantica pollen could be used as a proxy to reconstruct historic summer UV-B flux in Northwest Africa during at least the Holocene and Late Glacial period while also highlighting how UV-B proxies should be established using pollen samples from specimens growing in their native range or environment.

Highlights

Plants require sunlight to function, but too much exposure to ultraviolet-B radiation (UV-B, 280–315 nm) can have a damaging effect
THM-GC-MS analysis confirmed the presence of UV-B-absorbing compounds (UACs) para-coumaric acid (pCA) and ferulic acid (FA) in Cedrus atlantica pollen (Figure 2)
The chromatogram shows that FA concentrations in Cedrus atlantica pollen are higher than pCA concentrations, with an average of 2.5 times as much FA compared with pCA

Summary

Introduction

Plants require sunlight to function, but too much exposure to ultraviolet-B radiation (UV-B, 280–315 nm) can have a damaging effect. Plants have developed several repair and defence mechanisms to protect against UV-B exposure, including the production of UV-B-absorbing compounds (UACs). These compounds are present across different plant tissues, for example, epidermis layer, lignin, leaf tissues and pollen, and are thought to provide a screening mechanism to prevent UV-B radiation from penetrating into the mesophyll layers of plant leaves (Caldwell et al, 1998; Hollosy, 2002; Rozema et al, 1997; Sancar and Sancar, 1988; Taylor et al, 1997; Tevini et al, 1991). As changes in UV-B and ozone may affect atmospheric circulation and influence climate (Haigh, 2001; Rottman, 1999; Shindell et al, 2001), UV-B reconstructions could improve our understanding of the linkages between UV-B flux, ozone concentrations and climate change (Jardine et al, 2016; Magri, 2011)

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