UV 光の照射が生体外真皮モデルの材料特性に与える影響

Abstract

Recently, the skin health became one of the most important points for anti-aging treatment to improve QOL of aged people. The firmness of skin was maintained by collagen and elastin fibers secreted by fibroblasts in dermis layer. The wrinkles would be generated if these consistents of skin are degenerated. It is considered that the wrinkles are sometimes induced by ultraviolet A (UVA) irradiation to reach the dermis layer and cause the tissue degeneration, whereas the mechanism of wrinkles formation is still unclear. For this reason, many studies about the effect of UVA irradiation on the skin fibroblasts have been reported. However, the effect of that on material properties of skin tissue has not been studied. Therefore, the purpose of this study is to construct an in vitro skin model subjected to UVA irradiation and to evaluate the effect of UVA on the change in material properties of skin model. To simulate the dermis layer in vitro, human fibroblasts-populated collagen gels were constructed and subjected to the UVA irradiation before in vitro culture. After the culture, mechanical property of the specimens was evaluated. As the result, the elasticity of the reconstructed skin tissues differed depending on the UVA irradiation condition. From this result, it was suggested that material properties of the skin tissue changed depending on the UVA irradiation condition and these changes might be related to the wrinkle formation.