Abstract
Liquid and semi-solid culture media are used to maintain and proliferate bacteria, fungi, and Oomycetes for research in microbiology and plant pathology. In this study, a comparison was made between soybean milk medium, also referred to as soymilk, and media traditionally used for culturing soybean pathogens to determine if soymilk medium was an effective medium for growth of Colletotrichum truncatum, Fusarium virguliforme, Macrophomina phaseolina, Passalora sojina, Phomopsis longicolla, Phytophthora sojae, Pythium irregulare, Rhizoctonia solani, and Sclerotinia sclerotiorum. Based on radial mycelial colony growth rates, C. sojina grew significantly (P C. truncatum and F. virguliforme grew significantly (P P S. sclerotiorum grown on SDA as compared to PDA. Soymilk used with agar or used alone as a broth may be an option for replacing more expensive processed culture media.
Highlights
Culturing microbes dates back to the 19th century when, in 1876, Robert Koch used broths based on fresh beef serum or meat extracts to grow microorganisms [1,2]
All pathogens were cultured on potato dextrose agar (PDA) and potato dextrose broth (PDB) media, except P. sojina and P. sojae, which were maintained on V8 juice agar and V8 juice broth
The error variances of the area of colony growth per day of each pathogen were equal from the two trials (P > 0.05), and there was no interaction between the trial and treatment variables (P > 0.05)
Summary
Culturing microbes dates back to the 19th century when, in 1876, Robert Koch used broths based on fresh beef serum or meat extracts to grow microorganisms [1,2]. Two convenient and widely used commercial products for culturing soybean fungal pathogens are potato dextrose agar (PDA) and potato dextrose broth (PDB). These are powdered commercial media that can be made from basic ingredients. Media containing soybean products have been used to culture microbes including plant pathogens. The objectives were to 1) evaluate soymilk as a medium, in both broth and agar forms, to grow common soybean pathogens and 2) determine if any morphological structures were altered during growth on and in soymilk based media compared to media commonly used to grow these pathogens
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