Utilizing single primers as molecular markers inPoaspp.

Abstract

AbstractAn approach is described whereby single simple sequence repeat (SSR) primers are utilized at high annealing temperature to identify and evaluate the presence of palindrome or quasi‐palindrome regions in polyploid genomes to provide locus specific genotyping for polyploid grass species. The procedure has been effective in identifying markers that coexpress inPoa arachniferaand interspecificPoahybrids. DNA sequence analysis of the polymerase chain reaction (PCR) amplification products identified an array of homologous loci within and among evaluated individuals. From this study, 157 single primers were identified to provide information and identify polymorphisms acrossP. arachnifera. Similar to random amplified polymorphic DNA, arbitrarily primed PCR or low‐stringency single primer PCR methods, this approach requires no prior genome information, utilizes agarose gels and can be visualized with ethidium bromide. Preliminary evaluations of additionalPoaspp.,Bromus inermis,Dactylis glomerata,Thinopyrum ponticum,Lolium perenneandAgrostisspp. suggest wide utility of this approach toward genotyping polyploid grasses.