Abstract
Glycine N-methyltransferase is a tumor suppressor gene for hepatocellular carcinoma, which can activate DNA methylation by inducing the S-adenosylmethionine to S-adenosylhomocystine. Previous studies have indicated that the expression of Glycine N-methyltransferase is inhibited in hepatocellular carcinoma. To confirm and identify missing proteins, the pathologic analysis of the tumor-bearing mice will provide critical histologic information. Such a mouse model is applied as a screening tool for hepatocellular carcinoma as well as a strategy for missing protein discovery. In this study we designed an analysis platform using the human proteome atlas to compare the possible missing proteins to human whole chromosomes. This will integrate the information from animal studies to establish an optimal technique in the missing protein biomarker discovery.
Highlights
The completion of the human genome project that decoded more than 20,000 protein-coding genes has inspired enthusiastic efforts toward complete mapping of the human proteome to understand human biology
It consists of two major programs: the chromosome-based Human Proteome Project (HPP) (C-HPP) and the biology/disease HPP (BD-HPP) by which can expand our understanding of the human proteome of each gene on each chromosome and important biology/ disease-focused research
It has been indicated that Glycine N-methyltransferase (GNMT) expression was inhibited in hepatocellular carcinoma (HCC) and was a tumor susceptibility gene for liver cancer [21]
Summary
The completion of the human genome project that decoded more than 20,000 protein-coding genes has inspired enthusiastic efforts toward complete mapping of the human proteome to understand human biology. A*STAR’s Institute of Molecular and Cell Biology (IMCB) have shown new evidence that p21 activated protein kinases (PAK) affects a cancer associated protein that scientists have been studying for years. Their latest findings were published recently in Molecular Cell and have shown 4228 missing proteins in human chromosomes. The Human Proteome Project (HPP) is an international project organized by the Human Proteome Organization (HUPO), which is designed to map the entire human proteome in a systematic effort, using three major techniques: mass spectrometry (MS), antibodies (Abs), and the knowledgebase (KB) [4, 5] It consists of two major programs: the chromosome-based HPP (C-HPP) and the biology/disease HPP (BD-HPP) by which can expand our understanding of the human proteome of each gene on each chromosome and important biology/ disease-focused research.
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