Abstract
One titrimetric and two spectrophotometric methods which are simple, sensitive, and economic are described for the determination of sumatriptan succinate (STS) in bulk drug and in tablet dosage form using N-bromosuccinimide (NBS) as a brominating agent. In titrimetry, aqueous solution of STS is treated with a measured excess of NBS in acetic acid medium, and after the bromination of STS is judged to be complete, the unreacted NBS is determined iodometrically (method A). Spectrophotometric methods entail addition of a known excess of NBS in acid medium followed by the determination of residual NBS by its reaction with excess iodide, and the liberated iodine (I3 −) is either measured at 370 nm (method B) or liberated iodine is reacted with starch followed by the measurement of the blue colored starch-iodine complex at 570 nm (method C). Titrimetric method is applicable over range 1.0–10.0 mg STS (method A), and the reaction stoichiometry is found to be 1 : 3 (STS : NBS). The spectrophotometric methods obey Beer's law for concentration range 0.6–15.0 μg mL−1 (method B) and 0.2–4.0 μg mL−1 (method C). The calculated apparent molar absorptivity values were found to be 2.10 × 104 and 7.44 × 104 L mol−1 cm−1, for method B and method C, respectively.
Highlights
Triptans are a group of tryptamine-based drugs used in the acute treatment of migraine headaches
It is found that high performance liquid chromatography (HPLC) has been used for the assay of Sumatriptan succinate (STS) in human plasma [5, 6], human serum [7], rabbit plasma [8], and human plasma and urine [9] whereas liquid chromatography-mass spectrophotometry is used (LC-MS/MS) in body fluids [10] and human plasma [11]
Several methods have been reported for the determination of STS in pharmaceuticals and include UV-spectrophotometry [12,13,14,15,16], HPLC [17,18,19,20], ultra performance liquid chromatography (UPLC) [21], high performance thin layer chromatography (HPTLC) [16, 22], capillary electrophoresis [23], micellar electrokinetic chromatography [24], and voltammetry [25,26,27]
Summary
Triptans are a group of tryptamine-based drugs used in the acute treatment of migraine headaches. It is found that high performance liquid chromatography (HPLC) has been used for the assay of STS in human plasma [5, 6], human serum [7], rabbit plasma [8], and human plasma and urine [9] whereas liquid chromatography-mass spectrophotometry is used (LC-MS/MS) in body fluids [10] and human plasma [11]. Several methods have been reported for the determination of STS in pharmaceuticals and include UV-spectrophotometry [12,13,14,15,16], HPLC [17,18,19,20], ultra performance liquid chromatography (UPLC) [21], high performance thin layer chromatography (HPTLC) [16, 22], capillary electrophoresis [23], micellar electrokinetic chromatography [24], and voltammetry [25,26,27]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have