Utilization of Waste Mixed Pangasius Fish Fillet and Pineapple Core to Produce Peptone for Lactic Acid Bacteria Growth Media

Abstract

Over the last few decades, the quantum of research on probiotic strains has increased rapidly in most regions of the world. The use of probiotics in animal production and human nutrition can solve many problems caused by inadequate antibiotic treatment, growth promoters, disease prevention, and improved management. Market demand for probiotics is increasing worldwide, including Indonesia, where these are mainly imported. This situation renders peptone as a media component for isolation and identification of probiotics or as a media for testing bacteria in microbiological work. Pangasius fillet waste (P) and pineapple core (C) are agro-industrial wastes used as raw materials for peptone production. The purpose of this study was to see how the ratio of P to C affects the output of peptone from agro-industrial wastes for use as a component of laboratory media for the growth of microorganisms. Protein P hydrolysis was carried out directly by mixing C in various ratios (1:1, 2:1, and 3:1) with different incubation times (T), 1 day (T1), 2 days (T2), and 3 days (T3). The P: C ratio of 3:1 with an incubation time of one day (T1) was the best for peptone production, with a yield of 42.88±2.66%, the protein content of 5.68±0.13%, degree of hydrolysis of 90.16±2.28%, and 15 amino acids. The effects of commercial and modified MRS broth media containing peptone on LAB growth were non-significantly different, with an average total LAB of 9.084-9.128 log CFU/mL. In conclusion, peptone extracted from P using crude bromelain from C can be used as a nitrogen source to formulate MRS broth for LAB growth.