Utilization of Vegetable Waste Juice by Purple Non-Sulfur Bacterium (&amp;lt;i&amp;gt;Afifella marina&amp;lt;/i&amp;gt; Strain ME) for Biomass Production

Sujjat Al Azad,Wong Hao Jie,M T B M Lal

doi:10.4236/gep.2018.65017

Abstract

This experiment was conducted to determine the possibility of utilization of vegetable waste juice in the production of purple non-sulfur bacterium Afifella marina biomass. Bacterium was cultured in four diluted such as, 10%, 20%, 30% and 40% (v/v) vegetable waste juices. In addition synthetic 112 media were used as control to compare the growth characteristics of Afifella marine. The bacterium Afifella marina grew well in 10% diluted vegetable waste juice in anaerobic light condition with the highest production of 5.02 g/l dry cell weight in fifth day of culture, whereas the highest total carotenoid production of 0.87 mg/g dry cell weight was recorded in third day of culture. The highest dry cell weight productions recorded in 10% of VWJ was significantly higher compared to four other treatments (F = 14.63; p = 0.00). However, the total carotenoid production of bacteria in 10% VWJ showed no significant difference compared to the carotenoid production in 112 synthetic media, but was significantly higher compared to three other treatments (P = 0.00). The bacterium, Afifella marina has the ability to use vegetable waste juice at certain level for the production of bacterial biomass.

Highlights

Vegetable growing area in Sabah, Malaysia consists of around 3000 ha and most of the vegetables are produces in high land of Kundasang-Ranau area
The dry cell weight of Afifella marina cultured in both pure vegetable juice (PVJ) and the mixture of pure vegetable juice with yeast extract (PVJ + E) remained low with the production of bacterium biomass of 2.05 and 1.74 g/l respectively during the culturing period
The mean dry cell weight production recorded in the 112 media was significantly higher compare to both values recorded in Pure Vegetable Juice (PVJ) and PVJ + E (p = 0.00).there was no significant difference between the mean value recorded in PVJ and PVJ + E

Summary

Introduction

Vegetable growing area in Sabah, Malaysia consists of around 3000 ha and most of the vegetables are produces in high land of Kundasang-Ranau area. The waste contains, starch, cellulose, soluble sugar and organic acids which are utilized by microbes producing biomass of high protein content. The vegetable waste contains high calorific value and nutritive substrate to microbes [4] for the production of single-cell protein [5]. The organic matter from vegetable waste can be extracted for the use of single-cell protein cultivation after treatment. Solid state fermentation of vegetable waste using yeast is proven to increase the nutritive composition of crude protein with the value as high as 38% and 36% of crude lipid using yeast, Aspergillus niger [8]. No studies so far have been conducted with the utilization of waste vegetable juice as substrate for the production of Afifella marina biomass. This study was to determine the possibility of using PNSB, Afifella marine ME (KC205142) for the production of bacterium biomass using various concentration of vegetable juice as substrate

Methods

Results

Discussion

Conclusion