Utilization of trichoderma sp. and pseudomonas fluorescens as biofertilizer in shade-resistant soybean

Abstract

This study aims to obtain Trichoderma fungi and Pseudomonas fluorescens bacteria obtained from soybean planting fields and test their ability as biofertilizer agents to assist soybean growth until the end of the vegetative phase. From the soybean crop field, Trichoderma fungi and P. fluorescens (Pf) were isolated. After purification, observations were made to determine morphological physical characteristics both macroscopically and microscopically. Pf bacterial isolates were tested for their performance characteristics to ensure that bacteria were not pathogenic. For work power testing, as a biofertilizer agent, a factorial experiment was prepared in a completely randomized design (CRD). As the first factor, Trichoderma consists of without and with Trichoderma, while the second factor is Pf bacteria consisting of without and with Pf. The experiment was repeated four times so that 16 experimental units were obtained. The variables observed were plant height, number of leaves, stem diameter, and time of initial average flower appearance. Data were analyzed by ANOVA followed by HSD testing on a 5% test tarf. The results showed that the fungi Trichoderma sp. obtained by having single-celled, smooth-walled conidia measuring 3.2 x 2.0 µm, green with irregular upright hyaline conidiophores. As for P. fluorensence does not cause foul symptoms in test potatoes which are non-pathogenic. Fungi Trichoderma sp. TC-Jro-02 isolates and Pseudomonas fluorescens bacteria which were applied as biofertilizer agents were each increasing and inhibiting growth in plant height, leaf number, stover dry weight, and dry weight of soybean root of Dena varieties grown in shade up to 30 days after planting. Interactions between them affect the dry weight of stover and the dry weight of plant roots. The bacterial activity of Pseudomonas fluorescens inhibits the effect of the performance of Trichoderma fungi in supporting the growth of soybean plants in the shade.