Abstract
Large field of view (FOV) imaging was introduced and evaluated to characterize length and width of cellulose nanofibril (CNF) by transmission electron microscopy (TEM) with automation capability. New imaging method provided an image area as wide as covering one hole on grid. The large FOV image provided 10×10 of tile image which can cover 2500 μm² of sample area. Large FOV imaging has a great advantage to obtain massive information for average length of CNF. The imaging method enable to overcome the limitation of conventional TEM imaging to elicit average length of CNF. The average length and width of CNF was 375 nm and 7 nm, respectively. Length-weighted average length of CNF was 651 nm. Study to select the kind of support film resulted as carbon film was the best among silicon monoxide and carbon. Carbon film was more durable to high electron beam energy exposure and better to disperse CNF separately than silicon monoxide.
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