Utilization of pineapple peel and rice washing water to produce single cell proteins using Saccharomyces cerevisiae

Abstract

Single cell protein (SCP) is an alternative for meeting protein needs in the future. The wastes such as pineapple skin and rice water washing can be utilized to produce SCP. These two materials are abundant in Indonesia. The consumption of pineapple and rice in Indonesia is very high. The rice is the main food for Indonesian people. The aim of this study is to determine the best ratio of pineapple skin juice and rice washing water as a growing medium to produce single cell proteins. This study was performed in a completely randomized design with two factors. The first factor was the ratio of pineapple skin and rice washing water. The ratios between pineapple skin and rice washing water were 1: 1, 2: 1 and 1: 2. The second factor was fermentation times (8, 24, 32, 48, 56, 72 and 80 hours). The analysis carried out included pH, cell dry weight and protein content. The best treatment is obtained by the treatment of pineapple skin and rice washing water in ratio of 1: 2 when it is fermented for 56 hours. This media produced 0.4752 grams of cell dry weight with the protein content at about 289.08 ppm.