Utilization of Mycelium sterilium KT31 metabolites with diet for controlling Aeromonas hydrophila infection on catfish Clarias gariepinus

Abstract

Catfish (Clarias gariepinus) is a freshwater fish that is widely cultivated throughout Indonesia due to its importance commodity and high market prospect. Intensive aquaculture system to increase production has risks, including fish easily infected with the bacteria Aeromonas hydrophila or motile aeromonad septicaemia (MAS) disease in catfish. Alternative efforts to control MAS disease is by using natural source from marine fungal Mycelium sterilium KT31 metabolites. The purpose of this study was to determine the effective dose of fungal Mycelium sterilium KT31 metabolites in feed for preventing and treating infections caused by A. hydrophila in catfish. The feed was prepared by repelleting method and given restricted three times a day. The bacterial concentration used for the challenge test was density of 106 CFU/mL A. hydrophila as much as 0.1 mL per individual. This research was divided into two stage that the preventive experiment and treatment experiment with the different time challenge. Metabolites M. sterilium KT31 dose of 40 mL kg−1 of feed was able to preventing and treating A. hydrophila infection with a better blood value, resulting in the best survival rate and growth rate of catfish.