Abstract
In recent years measurement of permittivity signal has been increasingly used for online biomass monitoring of cell cultures. Breweries use it as an established method for fermenter inoculation and bioprocess control for instance [1]. In the case of animal cell cultures the correlation between permittivity and viable cell densities determined offline varies along cultivation time. Hence, several authors have used the permittivity signal as an indirect method for measuring oxygen and glutamine consumption as well as intracellular nucleotide phosphate concentrations [2,3]. The latest generation of biomass monitoring devices allows parallel measurement of permittivity at a range of frequencies leading to an improvement in the correlation between biomass and permittivity and providing a tool to further explore other aspects related to the physiological state of the cells.
Highlights
In recent years measurement of permittivity signal has been increasingly used for online biomass monitoring of cell cultures
A good correlation of the permittivity signal and the offline measured viable cell density for the growth phase was verified (R > 0.99), but pH-shifts and increased cell aggregation had a negative impact on the correlation
The linear factor to calculate the viable cell density from the online permittivity signal varied between 4.5·105 cells/(pF/cm) and 12.0 cells/(pF/cm)
Summary
In recent years measurement of permittivity signal has been increasingly used for online biomass monitoring of cell cultures. Breweries use it as an established method for fermenter inoculation and bioprocess control for instance [1]. Several authors have used the permittivity signal as an indirect method for measuring oxygen and glutamine consumption as well as intracellular nucleotide phosphate concentrations [2,3]. The latest generation of biomass monitoring devices allows parallel measurement of permittivity at a range of frequencies leading to an improvement in the correlation between biomass and permittivity and providing a tool to further explore other aspects related to the physiological state of the cells
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