Abstract
Lipase is an enzyme with numerous applications in industries as well as in environmental management. There are many lipolytic microorganisms and among them, fungal strains like Aspergillus and Penicillium are the prominent ones. Coastal environmental samples are good source for lipolytic fungi as they were exposed to oil spillages. Coastal soil samples were collected from Fort Kochi, Kerala and fungal isolates were isolated using serial dilution and spread plate method. Lipolytic fungal isolates were screened using phenol red based plate assay with olive oil as a substrate. Lipase assay was performed using titerometric method and a potential fungus was obtained which was identified through molecular methods as Penicillium citrinum. The optimization study revealed that dextrose and peptone were the best carbon and nitrogen sources. Similarly, medium pH of 7 and incubation temperature of 35 °C favoured the lipase production. Medicinal oil effluents from ayurvedic pharmaceuticals were obtained and studied for the suitability in lipase production under submerged conditions. About 20 % effluent with minimal medium could achieve maximum lipase activity of 266 U/ml using Penicillium citrinum MKF3. Hence, the present fungal isolate was found to be a promising lipase producer and suitable for managing oil rich effluents.
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