Abstract
A simple, novel, cost-effective and highly sensitive spectrofluorimetric method was developed for estimation of the nasal decongestant oxymetazoline (OMZ) whether per se or in its pharmaceutical preparations using colloidal silver nanoparticles (AgNPs). The method is based on the high catalytic potential activity of AgNPs on the fluorescence intensity of OMZ leading to 12-fold increase in its fluorescence intensity. The response was linear over the range of 20.0 to 700.0ng/mL with lower detection limit of 5.0ng/mL and limit of quantification of 14.0ng/mL. The proposed method was applied to the assay of commercial nasal drops, nasal spray and synthetic aqueous humor. Interference likely to be encountered from co-administered drugs was studied. The developed method was optimized and validated as per International Council of Harmonization (ICH). An explanation for the drug-AgNPs interaction was proposed.
Highlights
Nanoscience includes the study of the materials within nanoscale (1-100 nm)
A simple, novel, cost-effective and highly sensitive spectrofluorimetric method was developed for estimation of the nasal decongestant oxymetazoline (OMZ) whether per se or in its pharmaceutical preparations using colloidal silver nanoparticles (AgNPs)
In this study PVP was chosen as a stabilizer to prevent agglomeration of silver nanoparticles
Summary
Nanoscience includes the study of the materials within nanoscale (1-100 nm). Among the metal nanoparticles, AgNPs are the most widely used owing to their remarkable chemical and physical properties, including surface enhanced Raman scattering, optical action, electrical conductivity, high thermal, chemical stability and catalytic activity[1]. AgNPs have no fluorescence, they were frequently used in the spectrofluorometric analysis of some drugs in biological fluids and dosage forms They can cause either quenching or enhancement of the fluorescence of drug fluorophores and many drugs were determined based on this fact [2]. Localized surface plasmon resonance (LSPR) is a kind of surface plasmon excitations It occurs when light hits a nanoparticle having smaller size than the wavelength of the incident light. The reported spectrofluorimetric methods for OMZ are less sensitive, tedious and need chemical derivatization with either NBD-Cl reagent with linearity range (1–12 μg mL− 1) [19] or mucin with concentration range from (2.69 to 26.9 × 10− 5 mol L− 1) [20], while the proposed study which is based on its interaction with AgNPs leads to 12-fold increase of its fluorescence and a highly more sensitive method for its determination
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