Abstract
The lignin in PHL could be modified and recovered from pre-hydrolysis liquor (PHL) by horseradish peroxidase (HRP) treatment. The enzyme-modified lignin (EML) separated from PHL was used to adsorb and remove methylene blue (MB) from a model dye effluent; the adsorption performance of EML was studied. The results indicated the carboxyl content of the lignin in PHL increased by HRP treatment, and the EML removed from PHL had a carboxyl content of 0.69 mmol/g and a BET surface area of 25.6 m2/g. With the increase of pH, the adsorption capacity of EML obviously increased. The maximum MB adsorption capacity of EML was 210.2 mg/g and 241.1 mg/g at 298 K and 308 K, respectively. The fitting results revealed that the equilibrium adsorption isotherms and adsorption kinetics followed the Langmuir model and pseudo-second-order model, respectively.
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