Utilization of fish meal and fish oil for production of Cryptococcus sp. MTCC 5455 lipase and hydrolysis of polyurethane thereof.

Abstract

Fish meal has been used as an additional nitrogen source and fish oil as inducer for the growth and production of lipase from Cryptococcus sp. MTCC 5455. A response surface design illustrated that the optimum factors influencing lipase production were fish meal, 1.5%, w/v, Na2HPO4, 0.2%, w/v, yeast extract, 0.25%, w/v and sardine oil, 2.0%, w/v with an activity of 71.23U/mL at 96h and 25°C, which was 48.39% higher than the conventional one-factor-at-a-time method. The crude concentrated enzyme hydrolyzed polyurethane (PUR) efficiently and hydrolysis was 94% at 30°C and 96h. The products, diethylene glycol and adipic acid were quantified by HPLC and scanning electron microscopic studies of the degraded polymer showed significant increase in size of the holes from 24 to 72h of incubation. Hydrolysis of PUR within 96h makes the lipase novel for disposal of PUR and provides an innovative solution to the problems created by plastic wastes.