Abstract
Globally, dyes are widely used in the pharmaceutical, food, textile, cosmetics, plastics, leather, paint, ink and paper industries. Eosin is an acidic orange-pink dye and has very strong staining properties. Haematoxylin and eosin Y (H&E) combination is the most common staining and primary diagnostic technique in histo-pathological laboratories. This review mainly discussed the utility of eosin dye in quality control laboratories as an ion pairing agent for drug analysis. Eosin Y is one the most common ion pairing agent and its mono and di anionic forms of eosin Y are capable of interacting with many drug molecules to form colored/fluorescent binary or ternary complexes that can be analyzed with or without extraction by spectrofluorimetry and/or spectrophotometry. Quenching fluorescence and advantages of spectrofluorimetry over spectrophotometry were also discussed. Fluorescence detection greatly enhances the sensitivity and providing a sensitive and relatively inexpensive instrumental method of analysis using eosin Y for various important drugs in pure, commercial dosage forms and biological fluids
Highlights
Dyes are natural or synthetic colored organic substances which have the affinity to impart color to various substrates by absorbing into the substrate
Eosin dyes are bromine derivative of fluorescein which has two very closely related dyes commonly known as Eosin yellowish (Eosin Y) and Eosin bluish (Eosin B) as shown in fig
Eosin Y was used for determination of various pharmaceutical compounds using spectrophotometry and/or spectrofluorimetry through the formation of ternary complexes
Summary
Dyes are natural or synthetic colored organic substances which have the affinity to impart color to various substrates by absorbing into the substrate. Eosin Y is an acidic dye belongs to the xanthene group of dyes that has been widely used for the determination of several basic drugs through the formation of colored or fluorescent ion association complex using spectrophotometric and/or spectrofluorimetric methods. Binary ion association complexes formed between eosin and the drug molecules by electrostatic interaction.
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