Utilization of domestic wastewater as a water source of Tetradesmusobliquus PF3 for the biological removal of nitric oxide

Abstract

The reduction of nitrogen oxide (DeNOx) from flue gas by microalgae is a promising technology that has attracted increasing attention. Because the water source is a major limitation of microalgae application in the DeNOx from flue gas, we investigated the feasibility of using domestic wastewater (WW) as a water source. As a result, a biomass accumulation rate of 0.27 ± 0.01 mg L−1 d−1 was achieved by Tetradesmusobliquus PF3 cultivated in WW for 8 d, and 30 mg L−1 of nitrate nitrogen was added to the WW to fulfill the nutrient requirements of the microalgae cells. The ammonium (NH4+) nitrogen present in WW exerted inhibitory effects on the removal of nitric oxide (NO), thereby leading to 8% decrease removal efficiency in comparison with that using clean water and nutrients (BG11 medium). However, these inhibitory effects disappeared following the exhaustion of NH4+ by T. obliquus PF3 after 1 d. To overcome the inhibition of NH4+ and to achieve a high NO removal efficiency, a strategy of connecting two reactors in series was presented. The removal efficiency of NO by the two series reactors reached up to 71.2 ± 2.9%, which was significantly higher than that obtained by a single reactor (43.1 ± 3.6%). In addition, 70.9 ± 4.8% of the supplied NO was fixed into microalgae cells in the two reactors, which was 1.75 times higher than that in the single reactor (40.6 ± 5.1%), thereby suggesting that connecting two reactors in series rendered effective recovery of NO from flue gas using WW as a water source. In this study, we provided an economically viable water source for the application of microalgae in the biological DeNOx from flue gases.