Utilization of date products in production of oxytetracycline by Streptomyces rimosus

Abstract

Summary The components of the synthetic fermentation medium, especially carbon, nitrogen, and mineral sources were replaced by Barni date-coat (fruit-flesh) sugar extract, date-seed hydrolysate, date-seed lipid, and date-seed ash. Date-coat sugar extract was rich in sugars; while date-seed hydrolysate contained more nitrogen sources. Both date sources were utilized by the organism to give high titres of the antibiotic. The deficiency in nitrogen was overcome by the addition of urea. It was found that date-seed ash in concentration of 0.5 mg/ml could replace MgSO 4 , MnSO 4 , FeSO 4 , and ZnSO 4 when depleted from the medium. Combination of date sources (date-coat sugar extract, date-seed hydrolysate, date-seed lipid, and date-seed ash) was investigated to formulate a natural medium for formation of Oxytetracycline. The natural medium contained date sources was a convenient substrate for the biosynthesis of Oxytetracycline.