Abstract
Two new unicellular microalgal species from milk processing unit situated at Anand, India were screened and tested for their growth rate and nutrient removal capability under dairy wastewater at outdoor conditions in a simple column aeration photobioreactor. Results of 18s rRNA sequence analysis indicated that these strains have a close relationship with Chloromonas playfairii and Desmodesmus opoliensis named as SBC 2 and SBC 3, respectively. Both the strains removed more than 92% of ammonical nitrogen and 99% of total phosphorus, whereas COD and BOD reductions were found to be more than 95%. Maximum biomass production was 1.7 and 1.2 g L-1 and lipid content was 15 and 12%, respectively from SBC 2 and SBC 3 after 15 d cultivation. This study demonstrates the potential of algae based biofuel production by coupling wastewater treatment with microalgae cultivation for high lipid containing biomass production using Chloromonas playfairii and Desmodesmus opoliensis.Int J Appl Sci Biotechnol, Vol 3(4): 699-707
Highlights
Algae have recently received growing attention given its prospects as a source of renewable energy and its potential for CO2 capture
Isolation and Identification of Microalgae A total of 15 algal cultures were isolated from a dairy industry site, Anand, Gujarat, India by serial dilution method and were purified by plating and regular observation under microscope
Based on sequence data the two microalgal strains SBC 2 and SBC 3 were closely related to Chloromonas playfairii and Desmodesmus opoliensis based on 97 and 100% sequence similarities, respectively (Fig.1 & 2).The nucleotide sequence was submitted to NCBI database and the GenBank accession numbers for SBC 2 and SBC 3 are KT360945 and KT360946, respectively
Summary
Algae have recently received growing attention given its prospects as a source of renewable energy and its potential for CO2 capture. Due to the high cost and energy associated with chemical fertilizers as a source of nutrients, and given the relative unavailability of fresh water, inexpensive and accessible alternatives are necessary for sustainable microalgae based biofuels (Lam et al, 2012). Dairy wastewater is characterized by strong color, offensive odor, high BOD (40-48,000 mgL-1), high COD (80-95,000 mg L-1) Kushwaha et al, (2011) It contains sufficient amount of nitrogen and phosphorus for microalgal growth. The dairy wastewater is treated in anaerobic digesters to remove the high organic loads that are a potential source of organic carbons. These organic carbons are largely used by microalgae for their metabolic requirement (Wang et al, 2010)
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