Abstract

Two rat growth assays were conducted using chemically defined diets to determine the efficacy of D-methionine (MET) relative to L-MET. Experiment 1 established the linear growth response region for weanling rats fed a MET-free diet supplemented with graded levels of L-MET. Growth was linear when rats were fed L-MET levels between 0.10 and 0.25%. In experiment 2, the efficacy of D-MET was determined using the slope-ratio technique. Graded levels of D- and L-MET were fed to weanling rats, resulting in growth in the linear portion of the growth curve as established in experiment 1. Efficacy of D-MET was 88.5% of L-MET. Evaluation of urinary MET excretion revealed that only about 0.1% of the D-MET ingested was excreted via the kidneys.

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