Abstract

Abstract Endoglucanase production under submerged fermentation was studied using sugarcane bagasse (SCB) and oat bran (OB) as carbon source and corn steep liquor (CSL) as nitrogen source, in different concentrations using factorial design. Streptomyces diastaticus PA-01, isolated from a soil cave in Brazil, was selected as cellulolytic strain. The results after experimental validation showed that a medium containing 2.4% (w/v) SCB and 1.3% (w/v) CSL led to the highest production, 1,180.3 U.L-1 of endoglucanase, after the 5th-day. A good level of endoglucanase (1,039.3 U.L-1) was obtained after the 4th-day when 2.0% (w/v) OB and 1.65% (w/v) CSL were used. The pH and temperature profiles showed thermoacidophilic endoglucanase activity, with 70% of maximum activity at 50oC, after 4 hours of pre-incubation. This is the first report on endoglucanase production by S. diastaticus PA-01 in the presence of SCB and OB. The strong positive effects of some metal ions (Zn2+, Mn2+ and Ba2+) on endoglucanase activity when this strain was grown on OB is an interesting biochemical characteristic for future biotechnological applications.

Highlights

  • Brazil is one of the most prominent producers of lignocellulosic biomass, an abundant and renewable energy source

  • Actinobacterias are widely known for their cellulolytic potential and there are some reports in the literature about endoglucanase production (Jang and Chen, 2003; Grigorevski-Lima et al, 2005; Nascimento et al, 2009; Da Vinha et al, 2011; Franco-Cirigliano et al, 2013; Grigorevski-Lima et al, 2013)

  • Our results strongly suggest that the endoglucanases produced by Streptomyces diastaticus PA-01 in these supernatants (SCB and oat bran (OB) raw material) are thermo tolerant (> 70% for 4 hours at 50oC) and as such could be considered appropriate for some biotechnological processes, such as biomass hydrolysis for biorefinery purposes and industrial processes that demand long processing times at elevated temperatures, such as those in the food, sugar and fuel ethanol industries (Jang and Chen, 2003)

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Summary

Introduction

Brazil is one of the most prominent producers of lignocellulosic biomass, an abundant and renewable energy source. The utilization of lignocellulosic biomass (wheat bran, oat bran, sugarcane bagasse, sisal bagasse, etc.) as components of microbial growth media may represent cost reduction in the production of important. Oat bran is one of the most common agro-industrial byproducts used as raw material in several processes and products, mainly in formulated meat products to reduce total fat and sodium, but its acceptability as a source of increased dietary fiber has been limited. It consists mainly of xylans, cellulose, starch and protein (Dawkins et al 1999). Corn steep liquor, a major by-product of the corn wet-milling industry, is an inexpensive substrate available on a large scale, and has been shown to replace yeast extract very efficiently as a rich source of nutrients such as organic nitrogen and vitamins in microbial media (Akhtar et al, 1997; Nascimento et al, 2009)

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