Utilization of agro waste pectin for the production of industrially important polygalacturonase.

Abstract

In the present study, a variety of agro-industrial wastes have been utilized for meaningful purpose to produce valuable biocatalyst. All wastes used were low cost and easily accessible while, some available throughout the year. A number of bacterial strains isolated from rotten fruits and vegetables were screened for the production of industrially important polygalacturonase (PGase) using pectin present in these agro-industrial wastes. The strain producing maximum PGase was identified as Bacillus licheniformis KIBE-IB3 on the basis of taxonomic studies and 16S rDNA analysis. Among different agro-industrial wastes studied, high yield of PGase was achieved from fermentation broth having wheat bran (1.0%) as a substrate in to the medium supplemented with nitrogen sources in combination of NaNO3 and yeast extract while KH2PO4 was selected as suitable micronutrient. After optimizing fermentation parameters it was noticed that Bacillus licheniformis KIBE-IB3 was capable of producing maximum PGase at 37 °C, pH 7.0 and after 48 h of incubation time. From the current research, wheat bran was proven as a cheap and easily available source throughout the year for hyper production of pectinase. The utilization of the waste will also help to minimize the concerned environmental issues.