Abstract
The bacterial cellulose (BC) pellicle produced from Gluconacetobacter xylinus has many attractive properties to be used in food and biomedical applications. However, the by-product, gluconic acid produced during BC production will decrease the pH of culture that usually leads to a significant decrease of BC production by G. xylinus. To well control the pH of a static cultivation for BC pellicle production is not an easy task. In this study, acetate buffer was employed to maintain a pH suitable for BC production during static cultivation. The effect of buffer pH, ionic strength and surface area/volume (S/V) ratio of cultured medium on BC production was investigated. BC produced from 200 mM, pH 4.75 acetate buffered medium was 3.56 g/L, much higher than that obtained from YPD medium (0.66 g/L) and HS medium (1.23 g/L), respectively. When G. xylinus was cultivated at high glucose concentration (60 g/L) at S/V ratio of 0.55 cm−1, the BC produced and conversion were improved to 7.23 g/L and 0.2, respectively. As S/V ratio increased to 1.31 cm−1, a larger amount of gluconic acid (41.55 g/L) was obtained but with a slightly decreased BC production to 5.24 g/L. The results demonstrated that acetate buffered medium can effectively improve BC pellicle production in a static cultivation.
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