Utilization of a newly established gene isolation technique with metal coating hollow fiber membrane for viral gene collection

Abstract

Metal coating hollow fiber membrane (MCHF membrane) was made of gold metal plating on a polypropylene hollow fiber membrane. This membrane showed a trapping ability for microorganisms like as bacteria and it also can pass the high electric current. In this present report, we tried an isolation of viral gene employing MCHF membrane. MCHF membrane was used for a filter of viral suspension and it also used for an electrical wire of electrophoresis after filtration. Suspension of Herpes Simplex virus (HSV) was filtrated through the MCHF membrane and then the membrane was treated with a lyses solution (1% SDS in 10 mM NaOH). After these processes (about 15 min), the MCHF membrane was placed in the electrophoresis bath as a cathode. Viral gene was released from the MCHF membrane by applying electric current like as an electrophoresis, and the viral gene was accumulated using a semi-permeable membrane, which was placed among the electrodes. In this method, we could identify HSV gene that could be amplified by polymerase chain reaction (PCR). From these results, we considered that a newly established gene isolation technique with the MCHF membrane is a rapid and high performance gene collection method for the examination of virus-contaminated specimen such as blood products.