Utilization and growth response of Chrysosporum ovalisporum to different phosphorus compounds

Abstract

To explore the ability of bloom-forming cyanobacterium Chrysosporum ovalisporum to utilize different kinds of phosphorus compounds in the water column, we examined the growth response of C. ovalisporum in the laboratory by taking dipotassium hydrogen phosphate as the control and set different treatments of phosphorus substrates. The results showed that C. ovalisporum could utilize sodium tripolyphosphate and terasodium pyrophosphate decahydrate, with better utilization of sodium tripolyphosphate. After 15 days, it had the highest biomass and chlorophyll a concentrations under the treatment of sodium tripolyphosphate, with a value of (426.96±47.42) mg·L-1 and (1852.34±116.60) μg·L-1, respectively. Compared with the control, there was no significant difference in biomass of C. ovalisporum under both the (2-aminoethyl)-phosphonic acid and disodium β-glycerol phosphate pentahydrate treatments. The change characteristics of dissolved inorganic phosphate were related to the alkaline phosphatase activity, indicating that C. ovalisporum was able to utilize these two organophosphorus compounds via enzyme hydrolysis. The concentration of dissolved inorganic phosphate reached 0 mg·L-1 during the whole experiment when the C. ovalisporum were fed with glyphosate. Biomass, specific growth rate, chlorophyll a concentration and photosynthetic activity of algal cells were significantly lower than those of the control, indicating that C. ova-lisporum could not uptake phosphorus compounds in the glyphosate substrate and thus their growth being inhibited. Our results present new insights to understand the diffusion mechanism of C. ovalisporum into different aquatic ecosystems and had theoretical reference value for the prevention and control of new cyanobacterial blooms.