Utility of xpert® MTB/RIF ultra assay in the rapid diagnosis of bovine tuberculosis in wildlife and livestock animals from South Africa

Abstract

Members of the Mycobacterium tuberculosis complex (MTBC) bacteria, mainly Mycobacterium bovis (M. bovis), cause bovine tuberculosis (bTB) in livestock and wildlife animals. Confirmation of the disease is through culture and verification of the causative agent by molecular tests. In this study, we assessed the utility of the Xpert ® MTB/RIF Ultra assay, an automated molecular test originally designed to improve the detection of tuberculosis (TB) and rifampicin resistance in clinical sputum samples of human origin, by conducting a comparative evaluation with a culture based method routinely used at the Onderstepoort Veterinary Research (OVR). A total of 167 samples (tissue, n = 165; pus, n = 1; abscess, n = 1) from different wildlife and livestock animals (from 65 individual animals) were analyzed. Mycobacterium tuberculosis complex species was isolated in 63 (37.72 %) of the 167 samples, and was detected in 79 (47.3 %) of the samples by Xpert ® MTB/RIF Ultra assay. Based on the standard culture test, the diagnostic sensitivity and specificity of the Xpert ® MTB/RIF Ultra assay was found to be 95.24 % and 82 % respectively. All animals that were confirmed bTB positive by culture method were also found to be positive with the Xpert ® MTB/RIF Ultra assay in at least one sample (indicating a 100 % sensitivity of the method at the animal level). Non-tuberculous mycobacteria were isolated in 9 (3.4 %) of the samples analysed and none were detected by Xpert ® MTB/RIF Ultra assay, highlighting that this molecular test is highly specific. Xpert ® MTB/RIF Ultra assay was found to have great potential for the rapid diagnosis of the bTB in animals, hence allowing early intervention by regulatory authorities.